BCH 481 - Advanced Biochemistry I Lab

1 credit (Hrs: 3 Lab)

The Biochemistry Laboratory Course provides a focused exploration of proteins and their functions. Lab exercises will focus on the following: enzyme purification; enzyme kinetics; 1D, 2D and 3D NMR experiments and mass spectrometry experiments to explore the structure elucidation of oligopeptides; the use of ELISA to both identify the presence of proteins in a complex matrix and to determine whether or not a Natural Product can inhibit a specific protein; SDS-PAGE electrophoresis and Western Blot to study genetic relations in fish through analysis of myosin; bioinformatics studies of various proteins; transformation of E.Coli and isolation of new protein product; and an exploration of 3-dimensional shape of proteins using Protein Database and molecular modeling.

Corequisite(s): BCH 482. Course generally offered 2nd semester.