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Sep 07, 2024
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BCH 481 - Advanced Biochemistry I Lab1 credit (Hrs: 3 Lab) The Biochemistry Laboratory Course provides a focused exploration of proteins and their functions. Lab exercises will focus on the following: enzyme purification; enzyme kinetics; 1D, 2D and 3D NMR experiments and mass spectrometry experiments to explore the structure elucidation of oligopeptides; the use of ELISA to both identify the presence of proteins in a complex matrix and to determine whether or not a Natural Product can inhibit a specific protein; SDS-PAGE electrophoresis and Western Blot to study genetic relations in fish through analysis of myosin; bioinformatics studies of various proteins; transformation of E.Coli and isolation of new protein product; and an exploration of 3-dimensional shape of proteins using Protein Database and molecular modeling.
Corequisite(s): BCH 480 Course generally offered 2nd semester.
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